Application
(LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ETHE1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of 293 cells using ETHE1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ETHE1 Antibody in mouse liver tissue lysates (35ug/lane)| Product Name | ETHE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Persulfide dioxygenase ETHE1, mitochondrial, Ethylmalonic encephalopathy protein 1, Hepatoma subtracted clone one protein, Sulfur dioxygenase ETHE1, ETHE1, HSCO |
| Product description | ETHE1 is a sulfur dioxygenase that localizes within the mitochondrial matrix. The enzyme functions in sulfide catabolism. Mutations in its gene result in ethylmalonic encephalopathy.1) Tiranti,V., Nat. Med. 15 (2), 200-205 (2009) |
| Immunogen | This ETHE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-209 amino acids from the C-terminal region of human ETHE1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | ETHE1 |
|---|---|
| Alternative Names | Persulfide dioxygenase ETHE1 mitochondrial Ethylmalonic encephalopathy protein 1 Hepatoma subtracted clone one protein Sulfur dioxygenase ETHE1 ETHE1 HSCO |
| Molecular Weight(MW) | 28 kDa |
Application
(LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ETHE1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of 293 cells using ETHE1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ETHE1 Antibody in mouse liver tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
