As a transcriptional repressor; ETV7 binds to the DNA sequence 5'-CCGGAAGT-3'. But Isoform A and isoform C do not seem to have a repressor activity.1) Cardone, M., (2005) Mol. Cell. Biol. 25 (6), 2395-2405.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ETV7.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ETV7 antibody can be used for detection of ETV7 by ELISA at 1:1562500. ETV7 antibody can be used for detection of ETV7 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 0.0625 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
ETV7 antibody can be used for detection of ETV7 by ELISA at 1:1562500. ETV7 antibody can be used for detection of ETV7 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ETV7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.