Application
To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mExodus-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine Exodus-2 (XP-5129Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mExodus-2.| Product Name | Exodus-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLC, CKb9, TCA4, 6Ckine, Ccl21b, Scya21, Scya21b, Scya21c, exodus-2, C-C motif chemokine 21c |
| Product description | Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mExodus-2 (murine Exodus-2). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mExodus-2 (100.0 ng/mL), a concentration of 9.0-10.0 μg/mL of this antibody is required. ELISA: To detect mExodus-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mExodus-2. Sandwich: To detect mExodus-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Exodus-2 (XP-5129Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mExodus-2. Western Blot: To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Ccl21c |
|---|---|
| Alternative Names | SLC CKb9 TCA4 6Ckine Ccl21b Scya21 Scya21b Scya21c exodus-2 C-C motif chemokine 21c |
Application
To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mExodus-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine Exodus-2 (XP-5129Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mExodus-2.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mExodus-2 (100.0 ng/mL), a concentration of 9.0-10.0 μg/mL of this antibody is required. ELISA: To detect mExodus-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mExodus-2. Sandwich: To detect mExodus-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Exodus-2 (XP-5129Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mExodus-2. Western Blot: To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Exodus-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
