Application
Immunohistochemistry of FAF1 in rat spleen tissue with FAF1 antibody at 2.5 μg/mL.Application
Immunofluorescence of FAF1 in rat spleen tissue with FAF1 antibody at 2 μg/mL.Application
Western blot analysis of FAF1 in THP-1 cell lysate with FAF1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | FAF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FAF1 Antibody: Fas-associated protein 1 (FAF1) was initially identified as a Fas-binding pro-apoptotic protein that is component of the death-inducing signaling complex in Fas-mediated apoptosis. FAF1 can also induce apoptosis in the absence of extrinsic death signals when overexpressed although it does not contain typical death motifs such as the death domain, death effector domain, and caspase recruitment domain. Overexpression of FAF1 also decreases the basal level of NF-κB activity in transfected 293 cells, inhibits NF-κB activity induced by TNF-α, IL-1β and lipopolysaccharide, and prevents NF-κB translocation to the nucleus, suggesting that another role of FAF1 is to negatively regulate the activity of NF-κB. FAF1 can also interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway. Multiple differentially spliced isoforms of FAF1 are known to exist.1) Chu K, Niu X, and Williams LT. A Fas-associated protein factor, FAF1, potentiates Fas-mediated apoptosis. Proc. Natl. Acad. Sci. USA1995; 92:11894-8. |
| Immunogen | FAF1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAF1. The immunogen is located within the last 50 amino acids of FAF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FAF1 antibody can be used for detection of FAF1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAF1 |
|---|---|
| Alternative Names | FAF1 Antibody: hFAF1 CGI-03 HFAF1s UBXD12 UBXN3A FAS-associated factor 1 UBX domain-containing protein 12 hFAF1 |
| Molecular Weight(MW) | Predicted: 74 kDa Observed: 80 kDa |
| Sequence Similarities | 2) Cat. No. 1466 - Rat Spleen Tissue Lysate |
Application
Immunohistochemistry of FAF1 in rat spleen tissue with FAF1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of FAF1 in rat spleen tissue with FAF1 antibody at 2 μg/mL.
Application
Western blot analysis of FAF1 in THP-1 cell lysate with FAF1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1208 - THP-1 Cell Lysate |
|---|---|
| Application Notes | FAF1 antibody can be used for detection of FAF1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | FAF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FAF1 Antibody is supplied in PBS containing 0.02% sodium azide. |
