Application
Immunohistochemistry of FAM120A in rat brain tissue with FAM120A antibody at 2.5 μg/mL.Application
Western blot analysis of FAM120A in SK-N-SH cell lysate with FAM120A antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of FAM120A in Rat Brain cells with FAM120A antibody at 20 μg/mL.| Product Name | FAM120A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FAM120A Antibody: FAM120A (C9orf10) is a member of the constitutive coactivator of PPAR gamma family and the gene was mapped to chromosome 9q22.31. FAM120A was recently detected within the Pur-alpha-containing mRNA-protein complex in the brain. As a novel RNA-binding protein, FAM120A is a critical component of the oxidative stress-induced survival signaling. It may participate in mRNA transport in the cytoplasm. FAM120A activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. FAM120A protects cells from apoptosis through activation of SRKs in response to oxidative stress. Blocking of the survival signaling mediated by FAM120A, which sensitizes the cancer cells to stress-induced apoptosis, may be a novel therapeutic approach for gastric scirrhous carcinoma cells.1) Holden S and Raymond FL. The human gene CXorf17 encodes a member of a novel family of putative transmembrane proteins: cDNA cloning and characterization of CXorf17 and its mouse ortholog orf34. Gene2003; 318:149-61. |
| Immunogen | FAM120A antibody was raised against an 18 amino acid synthetic peptide from near the center of human FAM120A. The immunogen is located within amino acids 610 - 660 of FAM120A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FAM120A antibody can be used for detection of FAM120A by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAM120A |
|---|---|
| Alternative Names | FAM120A Antibody: OSSA C9orf10 DNAPTP1 DNAPTP5 KIAA0183 OSSA Constitutive coactivator of PPAR-gamma-like protein 1 Oxidative stress-associated Src activator |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | This antibody will not cross-reacti with FAM120B / PGCC1. |
Application
Immunohistochemistry of FAM120A in rat brain tissue with FAM120A antibody at 2.5 μg/mL.
Application
Western blot analysis of FAM120A in SK-N-SH cell lysate with FAM120A antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of FAM120A in Rat Brain cells with FAM120A antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | FAM120A antibody can be used for detection of FAM120A by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | FAM120A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FAM120A Antibody is supplied in PBS containing 0.02% sodium azide. |
