Application
Immunofluorescence of FAM193B in Jurkat cells with FAM193B antibody at 20 μg/mL.Application
Western blot analysis of FAM193B in Jurkat cell lysate with FAM193B antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | FAM193B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FAM193B Antibody: FAM193B, also known as IRIZIO, was initially identified as a protein that is upregulated in alveolar rhabdomyosarcoma (ARMS), a type of fast-growing tumor characterized by chromosomal translocations fusing the PAX3 or PAX7 gene with that of FOXO1. It has been suggested that, in addition to the PAX3-FOXO1 gene fusion, disruption of the Rb and p53 pathways is required for full ARMS tumorigenesis. In PAX3-FOXO1-expressing primary mouse fibroblasts that possessed a defective p53 pathway, FAM193B expression enabled the tumorigenic transformation, suggesting that FAM193B may contribute to rhabdomyosarcomagenesis in humans.1) Picchione F, Pritchard C, Lagutina I, et al. IRIZIO: a novel gene cooperating with PAX3-FOXO1 in alveolar rhabdomyosarcoma (ARMS). Carcinogenesis 2011; 32:452-62. |
| Immunogen | FAM193B antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human FAM193B. The immunogen is located within amino acids 120 - 170 of FAM193B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| FAM193B antibody can be used for detection of FAM193B by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAM193B |
|---|---|
| Alternative Names | FAM193B Antibody: IRIZIO KIAA1931 Protein FAM193B |
| Molecular Weight(MW) | 99 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | At least two isoforms of FAM193B are known to exist; this antibody will detect both isoforms. |
Application
Immunofluorescence of FAM193B in Jurkat cells with FAM193B antibody at 20 μg/mL.
Application
Western blot analysis of FAM193B in Jurkat cell lysate with FAM193B antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | FAM193B antibody can be used for detection of FAM193B by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | FAM193B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FAM193B Antibody is supplied in PBS containing 0.02% sodium azide. |
