Application
FAM44B Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of FAM44B Antibody in MCF-7 cell line lysates (35ug/lane)| Product Name | FAM44B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FAM44B required for proper chromosome biorientation through the detection or correction of syntelic attachments in mitotic spindles.1) Ling, H., et al. Obesity (Silver Spring) 17(4):737-744(2009) |
| Immunogen | This FAM44B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-155 amino acids from the C-terminal region of human FAM44B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BOD1 |
|---|---|
| Alternative Names | Biorientation of chromosomes in cell division protein 1 Biorientation defective protein 1 Protein FAM44B BOD1 FAM44B |
| Molecular Weight(MW) | 19 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
FAM44B Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of FAM44B Antibody in MCF-7 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
