Application
Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 μg/mL.Application
Immunofluorescence of FAM59A in Human Liver cells with FAM59A antibody at 20 μg/mL.Application
Western blot analysis of FAM59A in rat liver tissue lysate with FAM59A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | FAM59A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FAM59A Antibody: FAM59A, also known as GAREM, was initially identified through a mass spectroscoptic method that measures different phosphotyrosine states of proteins in response to different cell stimuli. FAM59A is a downstream molecule in the EGF signaling pathway that is tyrosine-phosphorylated, and this phosphorylation is needed for the binding of Grb2, an adaptor protein crucial to the transduction of growth signals from the plasma membrane to the nucleus. ERK activation in response to EGF stimulation is regulated by FAM59A in COS-7 and HeLa cells, and the overexpression of FAM59A stimulated cell proliferation and colony formation in soft agar, suggesting that FAM59A might be a critical protein with roles in ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells.1) Blagoev B, Ong SE, Kratchmarova I, et al. Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat. Biotechnol.2004; 22:1139-45. |
| Immunogen | FAM59A antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAM59A. The immunogen is located within amino acids 630 - 680 of FAM59A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FAM59A antibody can be used for detection of FAM59A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAM59A |
|---|---|
| Alternative Names | FAM59A Antibody: Gm944 FAM59A C18orf11 GRB2-associated and regulator of MAPK protein GRB2-associated and regulator of MAPK1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 μg/mL.
Application
Immunofluorescence of FAM59A in Human Liver cells with FAM59A antibody at 20 μg/mL.
Application
Western blot analysis of FAM59A in rat liver tissue lysate with FAM59A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | FAM59A antibody can be used for detection of FAM59A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | FAM59A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FAM59A Antibody is supplied in PBS containing 0.02% sodium azide. |
