Application
Western blot analysis of FAM59B in SK-N-SH cell lysate with FAM59B antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of FAM59B in Human Brain cells with FAM59B antibody at 20 μg/mL.Application
Immunohistochemistry of FAM59B in human brain tissue with FAM59B antibody at 5 μg/mL.| Product Name | FAM59B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FAM59B Antibody: FAM59B is related to the FAM59A protein (also known as GAREM). While little is known of the role of the FAM59B protein, FAM59A is a downstream molecule in the EGF signaling pathway and binds Grb2, an adaptor protein crucial to the transduction of growth signals from the plasma membrane to the nucleus. Like FAM59A, FAM59B may play a role in ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells.1) Blagoev B, Ong SE, Kratchmarova I, et al. Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat. Biotechnol.2004; 22:1139-45. |
| Immunogen | FAM59B antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAM59B. The immunogen is located within amino acids 600 - 650 of FAM59B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FAM59B antibody can be used for detection of FAM59B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAM59B |
|---|---|
| Alternative Names | FAM59B Antibody: FAM59B FAM59B KIAA2038 HRIHFB2063 GRB2-associated and regulator of MAPK protein-like GRB2-associated and regulator of MAPK1-like |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of FAM59B in SK-N-SH cell lysate with FAM59B antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of FAM59B in Human Brain cells with FAM59B antibody at 20 μg/mL.
Application
Immunohistochemistry of FAM59B in human brain tissue with FAM59B antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | FAM59B antibody can be used for detection of FAM59B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | FAM59B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FAM59B Antibody is supplied in PBS containing 0.02% sodium azide. |
