Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
FANCC Antibody IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Immunofluorescent analysis of U251 cells, using FANCC Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of lysates from HepG2,HL-60 cell line (from left to right),using FANCC Antibody .AP9522b was diluted at 1:1000 at each lane.| Product Name | FANCC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This protein is for complementation group C.1) Barroso, E., et al. Breast Cancer Res. Treat. 118(3):655-660(2009) |
| Immunogen | This FANCC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-555 amino acids from the C-terminal region of human FANCC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:25 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FANCC |
|---|---|
| Alternative Names | Fanconi anemia group C protein Protein FACC FANCC FAC FACC |
| Molecular Weight(MW) | 63 kDa |
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
FANCC Antibody IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Immunofluorescent analysis of U251 cells, using FANCC Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of lysates from HepG2,HL-60 cell line (from left to right),using FANCC Antibody .AP9522b was diluted at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:25 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
