Application
Western blot analysis of lysed extracts from HepG2, HeLa and 293 cells using Fas Antibody.| Product Name | Fas Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, RP11-399O19.9, IKBE, FASLG receptor, Apoptosis-mediating surface antigen FAS, Apo-1 antigen |
| Product description | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).1) Hahne, M. et al. (1995) Int Immunol 7, 1381-6. Nagata, S. (1997) Cell 88, 355-65. Green, D. R. and Ferguson, T. A. (2001) Nat Rev Mol Cell Biol 2, 917-24. |
| Immunogen | Fas antibody was raised against a peptide sequence around aa.323~327 (E-N-S-N-F) derived from human Fas . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAS |
|---|---|
| Alternative Names | APT1 CD95 FAS1 APO-1 FASTM ALPS1A TNFRSF6 RP11-399O19.9 IKBE FASLG receptor Apoptosis-mediating surface antigen FAS Apo-1 antigen |
| Molecular Weight(MW) | 40 - 50 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Fas protein. |
| Entrez Gene | 355 |
|---|---|
| Protein Accession | NP_000034.1 |
Application
Western blot analysis of lysed extracts from HepG2, HeLa and 293 cells using Fas Antibody.| Application Notes | Western Blot: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
