Application
Western blot analysis of FAS using rabbit polyclonal FAS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.Application
Confocal immunofluorescent analysis of FAS Antibody with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of FAS Antibody in T47D cell line lysates (35ug/lane)| Product Name | FAS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FAS is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells.1) Feig,C., et.al., EMBO J. 26 (1), 221-231 (2007) |
| Immunogen | This FAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-211 amino acids from the Central region of human FAS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAS |
|---|---|
| Alternative Names | Tumor necrosis factor receptor superfamily member 6 Apo-1 antigen Apoptosis-mediating surface antigen FAS FASLG receptor CD95 FAS APT1 FAS1 TNFRSF6 |
| Molecular Weight(MW) | 38 kDa |
Application
Western blot analysis of FAS using rabbit polyclonal FAS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.
Application
Confocal immunofluorescent analysis of FAS Antibody with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of FAS Antibody in T47D cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
