Application
Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 μg/mL.| Product Name | Fat Free Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Fat Free Antibody: Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.1) Perez-Victoria FJ, Schindler C, Magadan JG, et al. Ang2/Fat Free is a conserved subunit of the Golgi-associated retrograde protein complex. Mol. Biol. Cell 2010; 21:3386-95 |
| Immunogen | Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free. The immunogen is located within amino acids 620 - 670 of Fat Free. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Fat Free antibody can be used for detection of Fat Free by Western blot at 1 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C11orf2 |
|---|---|
| Alternative Names | Fat Free Antibody: FFR ANG2 ANG3 C11orf2 C11orf3 FFR PP5382 Vacuolar protein sorting-associated protein 51 homolog Another new gene 2 protein |
| Molecular Weight(MW) | 86 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms. |
Application
Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
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| Application Notes | Fat Free antibody can be used for detection of Fat Free by Western blot at 1 μg/mL. |
| Form | Liquid |
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| Storage Instructions | Fat Free antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Fat Free Antibody is supplied in PBS containing 0.02% sodium azide. |
