Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.| Product Name | FBL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FBL is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. FBL contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.1) Bouwmeester, T., (2004) Nat. Cell Biol. 6 (2), 97-105. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FBL. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| FBL antibody can be used for detection of FBL by ELISA at 1:12500. FBL antibody can be used for detection of FBL by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FBL |
|---|---|
| Alternative Names | FBL FIB FLRN RNU3IP1 |
| Molecular Weight(MW) | 35 kDa |
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | FBL antibody can be used for detection of FBL by ELISA at 1:12500. FBL antibody can be used for detection of FBL by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FBL antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
