Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the FBXL14 antibody detected the FBXL14 protein (arrow).| Product Name | FBXL14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FBXL14 is members of the F-box protein family, such as FBXL14, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.1) Vinas-Castells, R., et al. J. Biol. Chem. 285(6):3794-3805(2010) |
| Immunogen | This FBXL14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-407 amino acids from the C-terminal region of human FBXL14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FBXL14 |
|---|---|
| Alternative Names | F-box/LRR-repeat protein 14 F-box and leucine-rich repeat protein 14 FBXL14 FBL14 |
| Molecular Weight(MW) | 46 kDa |
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the FBXL14 antibody detected the FBXL14 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
