Application
Western blot analysis of lysate from MCF-7 cell line, using FBXL17 Isoform 2 Antibody at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded M. kidney section using FBXL17 Isoform 2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Immunohistochemical analysis of paraffin-embedded R. kidney section using FBXL17 Isoform 2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of MCF-7 cells using FBXL17 Isoform 2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.Application
Immunohistochemical analysis of paraffin-embedded H. kidney section using FBXL17 Isoform 2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.| Product Name | FBXL17 Isoform 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex (By similarity).1) Ota T.,et al.Nat. Genet. 36:40-45(2004). |
| Immunogen | This FBXL17 Isoform 2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 262-297 amino acids from the C-terminal region of human FBXL17 Isoform 2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For IHC-P starting dilution is: 1:25 For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FBXL17 |
|---|---|
| Alternative Names | F-box/LRR-repeat protein 17 F-box and leucine-rich repeat protein 17 F-box only protein 13 FBXL17 FBL17 FBX13 FBXO13 |
| Molecular Weight(MW) | 76 kDa |
Application
Western blot analysis of lysate from MCF-7 cell line, using FBXL17 Isoform 2 Antibody at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded M. kidney section using FBXL17 Isoform 2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Immunohistochemical analysis of paraffin-embedded R. kidney section using FBXL17 Isoform 2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of MCF-7 cells using FBXL17 Isoform 2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Application
Immunohistochemical analysis of paraffin-embedded H. kidney section using FBXL17 Isoform 2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:25 For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
