Application
Western blot analysis in mouse brain tissue and HepG2,293 cell line lysates (35ug/lane).Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FBXO2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is highly similar to the rat NFB42 (neural F Box 42 kDa) protein which is enriched in the nervous system and may play a role in maintaining neurons in a postmitotic state.1) Eom, C.Y., et al. Proc. Natl. Acad. Sci. U.S.A. 100(17):9803-9807(2003) Ilyin, G.P., et al. Gene 296 (1-2), 11-20 (2002)Yoshida, Y., et al. Nature 418(6896):438-442(2002) Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999) Cenciarelli, C., et al. Curr. Biol. 9(20):1177-1179(1999) |
| Immunogen | This FBXO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-271 amino acids from the C-terminal region of human FBXO2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FBXO2 |
|---|---|
| Alternative Names | F-box only protein 2 FBXO2 FBX2 |
| Molecular Weight(MW) | 33 kDa |
Application
Western blot analysis in mouse brain tissue and HepG2,293 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For FACS starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
