Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of FBXO3 Antibody in HepG2 cell line lysates (35ug/lane)| Product Name | FBXO3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FBXO3 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs.1) Shima,Y., et.al., Mol. Cell. Biol. 28 (23), 7126-7138 (2008) |
| Immunogen | This FBXO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-422 amino acids from the C-terminal region of human FBXO3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FBXO3 |
|---|---|
| Alternative Names | F-box only protein 3 FBXO3 FBX3 |
| Molecular Weight(MW) | 55 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of FBXO3 Antibody in HepG2 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
