Application
Antibody used in WB on Human U2OS at 5 ug/ml.Application
Antibody used in IP on Human U2OS at 5 ug/ml.Application
Antibody used in WB on Human MCF7 at 0.2-1 ug/ml.| Product Name | FBXO7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FBXO7 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXO7 belongs to the Fbxs class and it may play a role in regulation of hematopoiesis.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it may play a role in regulation of hematopoiesis. Alternatively spliced transcript variants of this gene have been identified with the full-length natures of only some variants being determined.1) Chang, Y.F., (2006) Biochem. Biophys. Res. Commun. 342 (4), 1022-1026. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FBXO7. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| FBXO7 antibody can be used for detection of FBXO7 by ELISA at 1:1562500. FBXO7 antibody can be used for detection of FBXO7 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FBXO7 |
|---|---|
| Alternative Names | FBXO7 DKFZp686B08113 FBX FBX07 FBX7 PKPS PARK15 |
| Molecular Weight(MW) | 58 kDa |
Application
Antibody used in WB on Human U2OS at 5 ug/ml.
Application
Antibody used in IP on Human U2OS at 5 ug/ml.
Application
Antibody used in WB on Human MCF7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | FBXO7 antibody can be used for detection of FBXO7 by ELISA at 1:1562500. FBXO7 antibody can be used for detection of FBXO7 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FBXO7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
