Application
Immunofluorescense staining of methanol - fixed Jurkat (top) and Raji (bottom) cells.Application
Western blot analysis using FES monoclonal antibody against truncated FES - His recombinant protein.| Product Name | FES Antibody [5A11G5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FES (feline sarcoma oncogene) and Fer are the only two members of a unique family of cytoplasmic protein tyrosine kinases.FES and Fer contain a central Src homology-2 (SH2) domain and a carboxy-terminal tyrosine kinase catalytic domain. They are structurally distinguished from other members of cytoplasmic protein tyrosine kinase subfamilies by the presence of amino-terminal Fer/CIP4 homology and coiled-coil domains. FES was originally identified as an oncogene from avian and feline retroviruses. Human c-Fes has been implicated in myeloid, vascular endothelial and neuronal cell differentiation. FES has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Mutations may activate the FES kinase and thereby contribute to cancer. However, recent data strongly suggests that the c-FES protein-tyrosine kinase is a tumor suppressor rather than a dominant oncogene in colorectal cancer.1) Delfino FJ. Stevenson H. Smithgall TE. J Biol Chem. 2006, Mar 31, 281(13): 8829-35. Epub 2006 Feb 2. |
| Immunogen | Ni-NTA purified truncated recombinant FES-His expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | FES |
|---|---|
| Alternative Names | FPS Proto-oncogene c-FesFPS |
| Sequence Similarities | 2) Cat. No. 1207 - Raji Cell Lysate |
Application
Immunofluorescense staining of methanol - fixed Jurkat (top) and Raji (bottom) cells.
Application
Western blot analysis using FES monoclonal antibody against truncated FES - His recombinant protein.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
| Form | Liquid |
|---|---|
| Storage Instructions | FES monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
