Application
Immunofluorescence of human brain tissue using FEZ2 antibody at 5 μg/mL.Application
Western blot analysis of FEZ2 in 3T3 cell lysate with FEZ2 antibody at (A) 0.5, and (B) 1 μg/mL.| Product Name | FEZ2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FEZ2 Antibody: Fasciculation and elongation protein zeta-2 (FEZ2) is a homolog to the mammalian FEZ1, itself an ortholog of the C. elegans UNC-76. In contrast to FEZ1, FEZ2 mRNA is widely expressed in mouse tissues. FEZ2 interacts with protein kinase C (PKC)-zeta substrate and induces neurite extension of PC12 cells when co-expressed with a constitutively active form of PKC-zeta, suggesting FEZ2 may play an important role in the morphological changes of various cells by associating with PKC-zeta in a tissue non-specific manner. FEZ2 can interact with FEZ1 through its c-terminal regions and especially its coiled-coil region. At least two isoforms of FEZ2 are known to exist.1) Fujita T, Ikuta J, Okajima T, et al. Identification of a tissue-non-specific homologue of axonal fasciculation and elongation protein zeta-1. Biochem. Biophys. Res. Commun.2004; 313:738-44. |
| Immunogen | FEZ2 antibody was raised against a 15 amino acid synthetic peptide from near the center of human FEZ2. The immunogen is located within amino acids 160 - 210 of FEZ2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| FEZ2 antibody can be used for the detection of FEZ2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunoflourescence starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FEZ2 |
|---|---|
| Alternative Names | FEZ2 Antibody: HUM3CL Fasciculation and elongation protein zeta-2 Zygin II |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Entrez Gene | 9637 |
|---|---|
| Protein Accession | NP_001036013 |
Application
Immunofluorescence of human brain tissue using FEZ2 antibody at 5 μg/mL.
Application
Western blot analysis of FEZ2 in 3T3 cell lysate with FEZ2 antibody at (A) 0.5, and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1212 - 3T3 Cell Lysate |
|---|---|
| Application Notes | FEZ2 antibody can be used for the detection of FEZ2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunoflourescence starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | FEZ2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FEZ2 Antibody is supplied in PBS containing 0.02% sodium azide. |
