Application
Western blot analysis of FFAR3 Antibody in Jurkat cell line lysates (35ug/lane)Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FFAR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FFAR3 is receptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation.1) Liaw, C.W., et al. DNA Cell Biol. 28(11):555-560(2009) |
| Immunogen | This FFAR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-322 amino acids from the C-terminal region of human FFAR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FFAR3 |
|---|---|
| Alternative Names | Free fatty acid receptor 3 G-protein coupled receptor 41 FFAR3 GPR41 |
| Molecular Weight(MW) | 39 kDa |
Application
Western blot analysis of FFAR3 Antibody in Jurkat cell line lysates (35ug/lane)
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
