Application
To detect mFGF-9 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine FGF-9 (XP-5135Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mFGF-9.| Product Name | FGF-9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mFGF-9 (murine FGF-9). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mFGF-9 (1.50 ng/mL), a concentration of 0.025 - 0.06 μg/mL of this antibody is required. ELISA: To detect mFGF-9 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mFGF-9. Sandwich: To detect mFGF-9 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine FGF-9 (XP-5135Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mFGF-9. Western Blot: To detect mFGF-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mFGF-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Fgf9 |
|---|---|
| Alternative Names | Eks Fgf-9 Fibroblast growth factor 9 Glia-activating factor FGF-9 |
Application
To detect mFGF-9 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine FGF-9 (XP-5135Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mFGF-9.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mFGF-9 (1.50 ng/mL), a concentration of 0.025 - 0.06 μg/mL of this antibody is required. ELISA: To detect mFGF-9 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mFGF-9. Sandwich: To detect mFGF-9 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine FGF-9 (XP-5135Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mFGF-9. Western Blot: To detect mFGF-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mFGF-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | FGF-9 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
