Application
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25-0.50 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. Tissue samples were provApplication
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25-0.50 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. Tissue samples were prov| Product Name | FGF-basic Antibody [20B242.2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hFGF-basic as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human FGF-basic when used with our biotinylated antigen affinity purified anti-human FGF-basic (XP-5137Bt) as the detection antibody at a concentration of approximately 0.125-0.25 μg/mL. Western blot: To detect hFGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-basic is 1.0-2.0 ng/lane, under non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FGF2 |
|---|---|
| Alternative Names | Fibroblast growth factor FGFB Fibroblast growth factor 2 Basic fibroblast growth factor FGF-2 BFGF HBGF-2 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25-0.50 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. Tissue samples were prov
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25-0.50 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. Tissue samples were prov| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human FGF-basic when used with our biotinylated antigen affinity purified anti-human FGF-basic (XP-5137Bt) as the detection antibody at a concentration of approximately 0.125-0.25 μg/mL. Western blot: To detect hFGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-basic is 1.0-2.0 ng/lane, under non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | FGF-basic lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
