Application
Western blot analysis of extracts from HepG2 cells treated with EGF using Phospho-FGFR1 (Tyr766) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | FGFR1 (phospho Tyr766) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.1) Cross MJ, et al. (2002) Mol Biol Cell 13, 2881-93. Foehr ED, Raffioni S, Murray-Rust J, Bradshaw RA (2001)J Biol Chem 276, 37529-36. Cross MJ, et al. (2000)J Cell Sci 113 ( Pt 4), 643-51 . |
| Immunogen | FGFR1 (phospho Tyr766) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 766 (Q-E-Y (p)-L-D) derived from FGFR1. |
| Modification | p-Tyr766 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FGFR1 |
|---|---|
| Alternative Names | FGFBR FGFR-1 FLG FLT2 c-fgr |
| Molecular Weight(MW) | 140 kDa |
| Tissue Specificity | The antibody detects endogenous level of FGFR1 only when phosphorylated at tyrosine 766. |
| Entrez Gene | 2260 |
|---|---|
| Protein Accession | NP_001167534.1 |
Application
Western blot analysis of extracts from HepG2 cells treated with EGF using Phospho-FGFR1 (Tyr766) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
