Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
FGFR1 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of FGFR1 Antibody in mouse liver tissue lysates (35ug/lane)| Product Name | FGFR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FGFR1 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction.1) Jiao, J., et al., Arch. Biochem. Biophys. 410(2):187-200 (2003). Fu, L., et al., J. Comp. Neurol. 462(2):265-273 (2003). Lundin, L., et al., Exp. Cell Res. 287(1):190-198 (2003). Kiselyov, V.V., et al., Structure (Camb.) 11(6):691-701 (2003). Baumann, H., et al., J. Biol. Chem. 278(18):16198-16208 (2003). |
| Immunogen | This FGFR1 antibody is generated from rabbits immunized with a His fusion protein from human FGFR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | FGFR1 |
|---|---|
| Alternative Names | Fibroblast growth factor receptor 1 FGFR-1 Basic fibroblast growth factor receptor 1 BFGFR bFGF-R-1 Fms-like tyrosine kinase 2 FLT-2 N-sam Proto-oncogene c-Fgr CD331 FGFR1 BFGFR CEK FGFBR FLG FLT2 HBGFR |
| Molecular Weight(MW) | 92 kDa |
Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
FGFR1 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of FGFR1 Antibody in mouse liver tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
