Application
Confocal immunofluorescent analysis of FIBG Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
FIBG Antibody IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of FIBG Antibody in NCI-H460 cell line lysates (35ug/lane)Application
Western Blot at 1:4000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | FIBG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FIBG is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in its gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.1) Nowak-Gottl,U., Blood 114 (9), 1947-1953 (2009) |
| Immunogen | This FIBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-449 amino acids from the C-terminal region of human FIBG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | FGG |
|---|---|
| Alternative Names | Fibrinogen gamma chain FGG |
| Molecular Weight(MW) | 52 kDa |
Application
Confocal immunofluorescent analysis of FIBG Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
FIBG Antibody IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of FIBG Antibody in NCI-H460 cell line lysates (35ug/lane)
Application
Western Blot at 1:4000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
