Application
Western blot analysis using anti - Human Fibulin 5 monoclonal antibody against truncated Fibulin 5 recombinant protein and Hela lysate.| Product Name | Fibulin5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DANCE, UNQ184/PRO210, Developmental arteries and neural crest EGF-like protein, FIBL-5, EVEC, UP50, ADCL2, ARMD3, DANCE, ARCL1A |
| Product description | Fibulin 5(FBLN5), with 448-amino acid protein (about 50 kDa), is a recently discovered multifunctional extracellular matrix protein that mediates endothelial cell adhesion through integrin ligation, regulates cell growth and motility in a context-specific manner, and prevents elastinopathy in vivo. Fibulin-5 is abundantly expressed in great vessels and cardiac valves during embryogenesis, and in many adult tissues including the aorta, lung, uterus and skin, all of which contain abundant elastic fibres. Decreased fibulin-5 may contribute to the pathogenesis of aortic dissection by impairing elastic fiber assembly. Fibulin-5 is also a good marker of skin ageing and that the earlier loss of fibulin-5 may involve age-dependent changes in other elastic fibre components.1) Wen Wang , Scott A. LeMaire, Li Chen. Surgery. 2005 Aug;138(2):352-9. |
| Immunogen | Ni-NTA purified truncated recombinant Fibulin 5 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:2000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | FBLN5 |
|---|---|
| Alternative Names | DANCE UNQ184/PRO210 Developmental arteries and neural crest EGF-like protein FIBL-5 EVEC UP50 ADCL2 ARMD3 DANCE ARCL1A |
Application
Western blot analysis using anti - Human Fibulin 5 monoclonal antibody against truncated Fibulin 5 recombinant protein and Hela lysate.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Fibulin 5 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
