Application
Immunohistochemistry of FKBP15 in mouse brain tissue with FKBP15 antibody at 2.5 μg/mL.Application
Immunofluorescence of FKBP15 in mouse brain tissue with FKBP15 antibody at 20 μg/mL.Application
Western blot analysis of FKBP15 in 3T3 cell lysate with FKBP15 antibody at 1 μg/mL.| Product Name | FKBP15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FKBP15 Antibody: FKBP15, also known as FKBP133, is a member of the FK506-binding protein family, a group of proteins initially identified as immunophilins, targets for the immunosupressant drugs FK506 and Rapamycin. FKBP15 is expressed in the developing nervous system and contains a domain similar to Wiskott-Aldrich syndrome protein homology region 1 (WH1) in addition to the FK506-binding protein motif. FKBP15 is distributed along the axonal shafts and partially co-localizes with F-actin in the growth cones of dorsal root ganglion neurons; overexpression of FKBP15 resulted in the number of filopodia in transfected neurons, suggesting that FKBP15 modulates growth cone behavior. FKBP15 has also been shown to associate with both microtubules and the actin filament systems and disruption of its expression by RNAi resulted in delayed transport of early endosomes in HeLa cells indicating that FKBP15 is also involved in the transport of early endosomes. At least three isoforms of FKBP15 are known to exist.1) Nakajima O, Nakamura F, Yamashita N, et al. FKBP133: A novel mouse FK506-binding protein homolog alters growth cone morphology. Biochem. Biophys. Res. Comm.2006; 346:140-9. |
| Immunogen | FKBP15 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FKBP15. The immunogen is located within amino acids 30 - 80 of FKBP15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FKBP15 antibody can be used for detection of FKBP15 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FKBP15 |
|---|---|
| Alternative Names | FKBP15 Antibody: FKBP133 PPP1R76 KIAA0674 FK506-binding protein 15 133 kDa FK506-binding protein FKBP-15 |
Application
Immunohistochemistry of FKBP15 in mouse brain tissue with FKBP15 antibody at 2.5 μg/mL.
Application
Immunofluorescence of FKBP15 in mouse brain tissue with FKBP15 antibody at 20 μg/mL.
Application
Western blot analysis of FKBP15 in 3T3 cell lysate with FKBP15 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | FKBP15 antibody can be used for detection of FKBP15 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | FKBP15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FKBP15 Antibody is supplied in PBS containing 0.02% sodium azide. |
