Application
Immunofluorescence staining of methanol-fixed HeLa cells using FLT3 (Ab-591).Application
Western blot analysis of lysed extracts from 293 and MCF cells using FLT3 (Ab-591).| Product Name | FLT3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLK2, STK1, CD135, FLK-2, CDKN6, FLT-3, FLT3, STK-1 |
| Product description | FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.1) Sekine S et, al. (2008) J Immunol. ;180 (12): 8126-34Pratz K et, al. (2008) Leuk Lymphoma. 2008 ;49 (5): 852-63. Al Shaer L et, al. (2008) Br J Haematol. 141 (4): 483-93. |
| Immunogen | FLT3 (Ab-591) antibody was raised against a peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FLT3 |
|---|---|
| Alternative Names | FLK2 STK1 CD135 FLK-2 CDKN6 FLT-3 FLT3 STK-1 |
| Molecular Weight(MW) | 130, 160 kDa |
| Tissue Specificity | This antibody detects endogenous level of total FLT3 protein. |
| Entrez Gene | 2322 |
|---|---|
| Protein Accession | NP_004110.2 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using FLT3 (Ab-591).
Application
Western blot analysis of lysed extracts from 293 and MCF cells using FLT3 (Ab-591).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
