Application
Western blot analysis of extracts from HepG2 cells treated with EGF using FLT3 (Phospho-Tyr842)) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | FLT3 (phospho Tyr842) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD135, STK1, FLK-2, FLT-3 |
| Product description | FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.1) Small D., Proc. Natl. Acad. Sci. U.S.A. 91:459-463(1994). |
| Immunogen | FLT3 (phospho Tyr842) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 842 (S-N-Y (p)-V-V) derived from Human FLT3 . |
| Modification | p-Tyr842 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FLT3 |
|---|---|
| Alternative Names | CD135 STK1 FLK-2 FLT-3 |
| Molecular Weight(MW) | 170 kDa |
| Tissue Specificity | The antibody detects endogenous levels of FLT3 only when phosphorylated at tyrosine 842. |
| Entrez Gene | 2322 |
|---|---|
| Protein Accession | NP_004110.2 |
Application
Western blot analysis of extracts from HepG2 cells treated with EGF using FLT3 (Phospho-Tyr842)) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
