| Product Name | flt3-Ligand Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FL, FLT3LFms-related tyrosine kinase 3 ligand, SL cytokine, Flt3 ligand |
| Product description | Flt3-Ligand is a growth factor important for the proliferation of hematopoietic cells. Flt3-Ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like Tyrosine Kinase-3 (FLT-3). Flt3-Ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt3-Ligand can stimulate the proliferation of cells expressing murine FLT-3 receptors. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hflt3-Ligand. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hflt3- Ligand (15 ng/mL), a concentration of 0.25 - 0.75 μg/mL of this antibody is required. ELISA: To detect hflt3-Ligand by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hflt 3-Ligand. Sandwich: To detect hFGF-basic by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FGF-basic (XP-5138Bt) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-basic. Western Blot: To detect hflt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hflt3-Ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FLT3LG |
|---|---|
| Alternative Names | FL FLT3LFms-related tyrosine kinase 3 ligand SL cytokine Flt3 ligand |
| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hflt3- Ligand (15 ng/mL), a concentration of 0.25 - 0.75 μg/mL of this antibody is required. ELISA: To detect hflt3-Ligand by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hflt 3-Ligand. Sandwich: To detect hFGF-basic by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FGF-basic (XP-5138Bt) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-basic. Western Blot: To detect hflt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hflt3-Ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | flt3-Ligand antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
