Application
Immunofluorescence of FOXA2 in human bladder tissue with FOXA2 antibody at 20 μg/ml.Application
Immunofluorescence of FOXA2 in mouse brain tissue with FOXA2 Antibodyat 20 μg/mL.Application
Immunohistochemistry of FOXA2 in mouse brain tissue with FOXA2 Antibodyat 5 μg/mL.Application
Western blot analysis of FOXA2 in human bladder tissue lysate with FOXA2 antibody at 1 μg/mL.| Product Name | FOXA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOXA2 Antibody: HNF3B, TCF3B, HNF3B, Hepatocyte nuclear factor 3-beta, Forkhead box protein A2, HNF-3-beta |
| Product description | FOXA2 Antibody: FOXA2 is one of three members of the FOXA family, a subset of the forkhead family of transcription factors which play vital roles in development. FOXA2 was initially identified through library screening as a closely related homolog of FOXA1. Both FOXA2 and FOXA1 act as transcriptional activators in adult liver and also play a role in body axis formation, neural tube patterning and definitive endoderm formation during gastrulation.1) Hannenhalli S and Kaestner KH. The evolution of Fox genes and their role in development and disease. Nat. Rev. Genet. 2009; 10:233-40. |
| Immunogen | FOXA2 antibody was raised against a 16 amino acid peptide near the center of human FOXA2 . The immunogen is located within amino acids 280 - 330 of FOXA2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FOXA2 Antibody can be used for detection of FOXA2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXA2 |
|---|---|
| Alternative Names | FOXA2 Antibody: HNF3B TCF3B HNF3B Hepatocyte nuclear factor 3-beta Forkhead box protein A2 HNF-3-beta |
| Molecular Weight(MW) | Predicted: 51 kDa Observed: 54 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (94%) |
| Tissue Specificity | At least two isoforms of FOXA2 are known to exist; this antibody will detect both isoforms. FOXA2 antibody is predicted to not cross-react with other members of the FOXA protein family. |
Application
Immunofluorescence of FOXA2 in human bladder tissue with FOXA2 antibody at 20 μg/ml.
Application
Immunofluorescence of FOXA2 in mouse brain tissue with FOXA2 Antibodyat 20 μg/mL.
Application
Immunohistochemistry of FOXA2 in mouse brain tissue with FOXA2 Antibodyat 5 μg/mL.
Application
Western blot analysis of FOXA2 in human bladder tissue lysate with FOXA2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1310 - Human Bladder Tissue Lysate |
|---|---|
| Application Notes | FOXA2 Antibody can be used for detection of FOXA2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FOXA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | FOXA2 Antibody is supplied in PBS containing 0.02% sodium azide. |
