Application
Immunofluorescence of FOXH1 in human liver tissue with FOXH1 antibody at 20 μg/mL.Application
Western blot analysis of FOXH1 in human liver tissue lysate with FOXH1 antibody at 1 μg/mL.| Product Name | FOXH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOXH1 Antibody: FAST1, FAST-1, FAST1, FAST2, Forkhead box protein H1, Forkhead activin signal transducer 1, Fast-1 |
| Product description | FOXH1 Antibody: FOXH1 is a member of the Forkhead-box (FOX) family of transcription factors and is the human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 plays an important role in mediating TGF-beta/activin signals through its interaction with the SMAD2-SMAD4 complex. It is expressed primarily during early development and localizes in the cytoplasm until it forms a complex with the SMAD proteins at which time it translocates to the nucleus to positively or negatively affect gene expression.1) Chen X, Weisberg E, Fridmacher V, et al. Smad and FAST-1 in the assembly of activin-responsive factor. Nature 1997; 389:85-9. |
| Immunogen | Rabbit polyclonal FOXH1 antibody was raised against a 19 amino acid peptide near the amino terminus of human FOXH1. The immunogen is located within the first 50 amino acids of FOXH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| FOXH1 antibody can be used for detection of FOXH1 by Western blot at 1:500~1:1000. For immunofluorescence start at 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXH1 |
|---|---|
| Alternative Names | FOXH1 Antibody: FAST1 FAST-1 FAST1 FAST2 Forkhead box protein H1 Forkhead activin signal transducer 1 Fast-1 |
| Molecular Weight(MW) | 40 kDa |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | FOXH1 antibody is predicted to not cross-react with any other members of the forkhead box family. |
Application
Immunofluorescence of FOXH1 in human liver tissue with FOXH1 antibody at 20 μg/mL.
Application
Western blot analysis of FOXH1 in human liver tissue lysate with FOXH1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | FOXH1 antibody can be used for detection of FOXH1 by Western blot at 1:500~1:1000. For immunofluorescence start at 1:50~1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | FOXH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FOXH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
