Foxh1 is a transcriptional activator. Foxh1 can recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3' and is required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Foxh1 forms a transcriptionally active complex containing FAST-1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).1) Georgiades, P. (2006) Development 133 (6), 1059-1068.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse FOXH1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
FOXH1 antibody can be used for detection of FOXH1 by ELISA at 1:62500. FOXH1 antibody can be used for detection of FOXH1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
FOXH1 antibody can be used for detection of FOXH1 by ELISA at 1:62500. FOXH1 antibody can be used for detection of FOXH1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store FOXH1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.