Application
Immunofluorescence of FOXRED2 in human lung cells with FOXRED2 antibody at 20 μg/mL.Application
Western blot analysis of FOXRED2 in human lung tissue lysate with FOXRED2 antibody at 1 μg/mL.| Product Name | FOXRED2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOXRED2 Antibody: ERFAD, ERFAD, FAD-dependent oxidoreductase domain-containing protein 2, Endoplasmic reticulum flavoprotein associated with degradation |
| Product description | FOXRED2 Antibody: FOXRED2, also known as ERFAD, is an endoplasmic reticulum (ER) luminal flavoprotein which may function in ER associated degradation (ERAD). It has been proposed to bind non-native proteins in the endoplasmic reticulum and target them to the ubiquitination machinery for subsequent degradation. Upon knockdown of FOXRED2, the degradation of the ERAD model substrate ribophorin 332 is delayed, and the overall level of polyubiquitinated cellular proteins is decreased. Defects in the related protein FOXRED1 are a cause of mitochondrial complex I deficiency (MT-C1D), suggesting the FOXRED2 may also play a role in MT-C1D.1) Riemer J, Appenzeller-Herzog C, Johansson L, et al. A luminal flavoprotein in endoplasmic reticulum-associated degradation. Proc. Natl. Acad. Sci. USA 2009; 106:14831-6. |
| Immunogen | FOXRED2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human FOXRED2. The immunogen is located within amino acids 70 - 120 of FOXRED2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| FOXRED2 antibody can be used for detection of FOXRED2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXRED2 |
|---|---|
| Alternative Names | FOXRED2 Antibody: ERFAD ERFAD FAD-dependent oxidoreductase domain-containing protein 2 Endoplasmic reticulum flavoprotein associated with degradation |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (94%) |
| Entrez Gene | 80020 |
|---|---|
| Protein Accession | No. Q8IWF2 |
Application
Immunofluorescence of FOXRED2 in human lung cells with FOXRED2 antibody at 20 μg/mL.
Application
Western blot analysis of FOXRED2 in human lung tissue lysate with FOXRED2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | FOXRED2 antibody can be used for detection of FOXRED2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FOXRED2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FOXRED2 Antibody is supplied in PBS containing 0.02% sodium azide. |
