Application
Formalin-fixed and paraffin-embedded human lung carcinoma with FPGS Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of K562 cells using FPGS Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of FPGS Antibody in K562, A2058 cell line lysates (35ug/lane)| Product Name | FPGS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Folylpolyglutamate synthase, mitochondrial, Folylpoly-gamma-glutamate synthetase, FPGS, Tetrahydrofolylpolyglutamate synthase, Tetrahydrofolate synthase, FPGS |
| Product description | FPGS is the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives.1) Sharma,S., et.al., Pharmacogenet. Genomics 18 (12), 1041-1049 (2008) |
| Immunogen | This FPGS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the Central region of human FPGS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FPGS |
|---|---|
| Alternative Names | Folylpolyglutamate synthase mitochondrial Folylpoly-gamma-glutamate synthetase FPGS Tetrahydrofolylpolyglutamate synthase Tetrahydrofolate synthase FPGS |
| Molecular Weight(MW) | 65 kDa |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with FPGS Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of K562 cells using FPGS Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of FPGS Antibody in K562, A2058 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
