Application
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.Application
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.Application
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1μg/mL.| Product Name | FREM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FREM1 Antibody: BNAR, MOTA, TILRR, TRIGNO2, C9orf143, C9orf145, C9orf154, FRAS1-related extracellular matrix protein 1, Protein QBRICK |
| Product description | FREM1 Antibody: FREM1 is a member of the FRAS1-related extracellular matrix protein family and is thought to play a role in craniofacial and renal development. FREM1 functions as an extracellular matrix protein that is essential for epidermal adhesion during embryogenesis and may also participate in epidermal differentiation. It is recognized by cells in the embryonic skin and hair follicles through different members of the integrin family. Deficiency in the Fras1/Frem genes gives rise to the bleb phenotype, which is equivalent to the human hereditary disorder Fraser syndrome.1) Smyth I, Du X, Taylor MS, et al. The extracellular matrix gene FREM1 is essential for the normal adhesion of the embryonic epidermis. Proc. Natl. Acad. Sci. USA2004; 101:13560-5. |
| Immunogen | FREM1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human FREM1. The immunogen is located within the last 50 amino acids of FREM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| FREM1 antibody can be used for detection of FREM1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FREM1 |
|---|---|
| Alternative Names | FREM1 Antibody: BNAR MOTA TILRR TRIGNO2 C9orf143 C9orf145 C9orf154 FRAS1-related extracellular matrix protein 1 Protein QBRICK |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.
Application
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.
Application
Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | FREM1 antibody can be used for detection of FREM1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FREM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FREM1 Antibody is supplied in PBS containing 0.02% sodium azide. |
