Application
Immunofluorescence of FREM2 in A20 cells with FREM2 antibody at 20 μg/mL.Application
Immunocytochemistry of FREM2 in A-20 cells with FREM2 antibody at 20 μg/mL.Application
Western blot analysis of FREM2 in A-20 cell lysate with FREM2 antibody at (A) 0.5 and (B) 1μg/mL.| Product Name | FREM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FREM2 Antibody: FRAS1-related extracellular matrix protein 2, ECM3 homolog |
| Product description | FREM2 Antibody: FREM2 is a member of the FRAS1-related extracellular matrix protein family and like FREM1, mutations in the FREM2 gene are associated with Fraser syndrome. FREM2 localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions during morphogenetic processes and is thought to be required for maintaining the integrity of the skin epithelium and the differentiated state of renal epithelia. The FREM2 gene is one of several genes whose transcription is affecteded by TFAP2C, a transcription factor involved in mammary development, differentiation, and oncogenesis.1) Jadjeda S, Smyth I, Pitera JE, et al. Identification of a new gene mutated in Fraser syndrome and mouse myelencephalic blebs. Nat. Genet.2005; 37:520-5. |
| Immunogen | FREM2 antibody was raised against a 19 amino acid synthetic peptide near the center of human FREM2. The immunogen is located within amino acids 1920 - 1970 of FREM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| FREM2 antibody was raised against a 19 amino acid peptide near the center of human FREM2. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FREM2 |
|---|---|
| Alternative Names | FREM2 Antibody: FRAS1-related extracellular matrix protein 2 ECM3 homolog |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
Application
Immunofluorescence of FREM2 in A20 cells with FREM2 antibody at 20 μg/mL.
Application
Immunocytochemistry of FREM2 in A-20 cells with FREM2 antibody at 20 μg/mL.
Application
Western blot analysis of FREM2 in A-20 cell lysate with FREM2 antibody at (A) 0.5 and (B) 1μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | FREM2 antibody was raised against a 19 amino acid peptide near the center of human FREM2. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FREM2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FREM2 Antibody is supplied in PBS containing 0.02% sodium azide. |
