Application
Western blot analysis of FRMPD1 in K562 cell lysate with FRMPD1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of FRMPD1 in K562 cells with FRMPD1 antibody at 20 μg/mL.| Product Name | FRMPD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FRMPD1 Antibody: FRMD2, FRMD2, KIAA0967, FERM and PDZ domain-containing protein 1, FERM domain-containing protein 2 |
| Product description | FRMPD1 Antibody: The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD1 was initially identified through a yeast two-hybrid screening with Activator of G-protein signaling 3 (AGS3), a protein that acts to stabilize the GDP-bound conformation of Gai, as the bait. FRMPD1 binds to the tetratricopeptide repeat of AGS3 and stabilizes AGS3 in a membrane environment. Suppression of FRMPD1 in Cath.a-differentiated neuronal cells decreased the level of endogenous AGS3 in membrane fractions by ~50% and enhanced the inhibition of forskolin-induced increases in cAMP. Co-immunoprecipitation studies indicate that the interaction of AGS3 with FRMPD1 is mutually exclusive with the interaction of AGS3 with Gai3, suggesting that FRMPD1 may position AGS3 in the membrane where it can then interact with Galphai.1) An N, Blumer JB, Bernard ML. The PDZ and Band 4.1 containing protein Frmpd1 regulates the subcellular location of activator of G-protein signaling 3 and its interaction with G-proteins. J. Biol. Chem.2008; 283:24718-28. |
| Immunogen | FRMPD1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human FRMPD1. The immunogen is located within amino acids 1330 - 1380 of FRMPD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| FRMPD1 antibody can be used for detection of FRMPD1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FRMPD1 |
|---|---|
| Alternative Names | FRMPD1 Antibody: FRMD2 FRMD2 KIAA0967 FERM and PDZ domain-containing protein 1 FERM domain-containing protein 2 |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Western blot analysis of FRMPD1 in K562 cell lysate with FRMPD1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of FRMPD1 in K562 cells with FRMPD1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | FRMPD1 antibody can be used for detection of FRMPD1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FRMPD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FRMPD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
