Application
Immunohistochemistry of FRMPD2 in mouse kidney tissue with FRMPD2 antibody at 5 μg/mL.Application
Western blot analysis of FRMPD2 in rat kidney tissue lysate with FRMPD2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | FRMPD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FRMPD2 Antibody: PDZK4, PDZD5C, PDZK5C, PDZK4, FERM and PDZ domain-containing protein 2, PDZ domain-containing protein 4 |
| Product description | FRMPD2 Antibody: The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD2 also contains an N-terminal KIND domain and three PDZ domains and is structurally similar to the protein tyrosine phosphatase PTP-BL. FRMPD2 is localized in a polarized fashion in epithelial cells at the basolateral membrane and partially co-localizes with the tight-junction marker protein Zonula-occuldens-1. Suppression of FRMPD2 expression via RNAi in Caco-2 cells results in an impairment of tight junction formation, indicating that FRMPD2 plays a major role in tight junction formation. Other experiments indicate that FRMPD2 is a binding partner to several catenin family members and recruitment of FRMPD2 to cell-cell contacts is dependent on E-cadherin-mediated cell-cell adhesion.1) An N, Blumer JB, Bernard ML. The PDZ and Band 4.1 containing protein Frmpd1 regulates the subcellular location of activator of G-protein signaling 3 and its interaction with G-proteins. J. Biol. Chem.2008; 283:24718-28. |
| Immunogen | FRMPD2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FRMPD2. The immunogen is located within amino acids 80 - 130 of FRMPD2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| FRMPD2 antibody can be used for detection of FRMPD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FRMPD2 |
|---|---|
| Alternative Names | FRMPD2 Antibody: PDZK4 PDZD5C PDZK5C PDZK4 FERM and PDZ domain-containing protein 2 PDZ domain-containing protein 4 |
| Entrez Gene | 143162 |
|---|---|
| Protein Accession | NP_001018081 |
Application
Immunohistochemistry of FRMPD2 in mouse kidney tissue with FRMPD2 antibody at 5 μg/mL.
Application
Western blot analysis of FRMPD2 in rat kidney tissue lysate with FRMPD2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1465 - Rat Kidney Tissue Lysate |
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| Application Notes | FRMPD2 antibody can be used for detection of FRMPD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | FRMPD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FRMPD2 Antibody is supplied in PBS containing 0.02% sodium azide. |
