Application
Immunohistochemistry of FRMPD4 in human brain tissue with FRMPD4 antibody at 5 μg/mL.Application
Immunofluorescence of FRMPD4 in human brain tissue with FRMPD4 antibody at 20 μg/mL.Application
Western blot analysis of FRMPD4 in SK-N-SH cell lysate with FRMPD4 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | FRMPD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FRMPD4 Antibody: TMEM94, TMEM94, Uncharacterized protein KIAA0195, Transmembrane protein 94 |
| Product description | FRMPD4 Antibody: The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD4, also known as Preso, also contains another protein interaction domain termed WW (domain with two conserved Trp residues) at its amino terminus. It was identified through a yeast two-hybrid screen using the PDZ domain of PSD-95 as bait and is highly expressed in multiple regions of the brain and is enriched in the postsynaptic density (PSD) fractions. Overexpression of FRMPD4 in cultured hippocampal neurons significantly increased the linear density of dendritic spines without changing their length and width; conversely, knockdown experiments using RNAi caused a decrease in spine density, indicating FRMPD4 positively regulates dendritic spine density but not morphology. The decreased level of FRMPD4 also resulted in reduced levels of excitatory synaptic transmission, suggesting that FRMPD4 is required for maintenance of excitatory synaptic transmission.1) An N, Blumer JB, Bernard ML. The PDZ and Band 4.1 containing protein Frmpd1 regulates the subcellular location of activator of G-protein signaling 3 and its interaction with G-proteins. J. Biol. Chem.2008; 283:24718-28. |
| Immunogen | FRMPD4 antibody was raised against an 18 amino acid synthetic peptide from near the center of human FRMPD4. The immunogen is located within amino acids 620 - 670 of FRMPD4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FRMPD4 antibody can be used for detection of FRMPD4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIAA0195 |
|---|---|
| Alternative Names | FRMPD4 Antibody: TMEM94 TMEM94 Uncharacterized protein KIAA0195 Transmembrane protein 94 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of FRMPD4 in human brain tissue with FRMPD4 antibody at 5 μg/mL.
Application
Immunofluorescence of FRMPD4 in human brain tissue with FRMPD4 antibody at 20 μg/mL.
Application
Western blot analysis of FRMPD4 in SK-N-SH cell lysate with FRMPD4 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | FRMPD4 antibody can be used for detection of FRMPD4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FRMPD4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FRMPD4 Antibody is supplied in PBS containing 0.02% sodium azide. |
