Application
Immunofluorescence of FXYD4 in rat lung tissue with FXYD4 antibody at 20 μg/mL.Application
Immunohistochemistry of FXYD7 in rat lung tissue with FXYD7 antibody at 2.5 μg/mL.Application
Western blot analysis of FXYD7 in human lung tissue lysate with FXYD7 antibody at 1 μg/mL.| Product Name | FXYD7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FXYD7 Antibody: FXYD domain-containing ion transport regulator 7 |
| Product description | FXYD7 Antibody: FXYD7 is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing seven invariant and six highly conserved amino acids. The FXYD proteins are tissue-specific regulators of Na, K-ATPase, with FXYD7 initially identified as a brain-specific member. FXYD7 interacts with Na, K-ATPase through its transmembrane domain and is thought to influence the affinity of Na, K-ATPase for external K+ and Na+ ions. Other members of the FXDY family have similar functions: FXYD2 regulates the properties of Na, K-ATPase, while FXYD1 (phospholemman), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems.1) Beguin P, Crambert G, Monnet-Tschudi F, et al. FXYD7 is a brain-specific regulator of Na,K-ATPase alpha1-beta isozymes. EMBO J. 2002; 21:3264-73 |
| Immunogen | FXYD7 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human FXYD7. The immunogen is located within the first 50 amino acids of FXYD7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FXYD7 antibody can be used for detection of FXYD7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FXYD7 |
|---|---|
| Alternative Names | FXYD7 Antibody: FXYD domain-containing ion transport regulator 7 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (71%) |
| Tissue Specificity | FXYD7 antibody is human, mouse and rat reactive. FXYDY7 antibody is predicted to not react with other members of the FXDY protein family |
Application
Immunofluorescence of FXYD4 in rat lung tissue with FXYD4 antibody at 20 μg/mL.
Application
Immunohistochemistry of FXYD7 in rat lung tissue with FXYD7 antibody at 2.5 μg/mL.
Application
Western blot analysis of FXYD7 in human lung tissue lysate with FXYD7 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | FXYD7 antibody can be used for detection of FXYD7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FXYD7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FXYD7 Antibody is supplied in PBS containing 0.02% sodium azide. |
