Application
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Product Name | G3BP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G3BP, G3BP, HDH-VIII |
| Product description | G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.1) Kociok, N., et al., (1999) J. Cell. Biochem. 74 (2), 194-201. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human G3BP. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| G3BP antibody can be used for detection of G3BP by ELISA at 1:312500. G3BP antibody can be used for detection of G3BP by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | G3BP1 |
|---|---|
| Alternative Names | G3BP G3BP HDH-VIII |
| Molecular Weight(MW) | 51 kDa, 52 kDa |
Application
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | G3BP antibody can be used for detection of G3BP by ELISA at 1:312500. G3BP antibody can be used for detection of G3BP by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store G3BP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
