Application
G3BP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Product Name | G3BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ras GTPase-activating protein-binding protein 1, G3BP-1, ATP-dependent DNA helicase VIII, hDH VIII, GAP SH3 domain-binding protein 1, G3BP1, G3BP |
| Product description | This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.1) Gao, X., et al. FEBS Lett. 584(16):3525-3532(2010) |
| Immunogen | This G3BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the C-terminal region of human G3BP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | G3BP1 |
|---|---|
| Alternative Names | Ras GTPase-activating protein-binding protein 1 G3BP-1 ATP-dependent DNA helicase VIII hDH VIII GAP SH3 domain-binding protein 1 G3BP1 G3BP |
| Molecular Weight(MW) | 52 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
G3BP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
