Application
GABA A Receptor alpha 6 Antibody| Product Name | GABA A Receptor alpha 6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA-Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha and beta subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha subunits of the receptor. Lastly, phosphorylation of beta subunits of the receptor has been shown to modulate GABAA-R function.1) Kittler, J.T., McAinsh, K., Moss, S.J. "Mechanisms of GABA A receptor assembly and trafficking - Implications for the modulation of inhibitory neurotransmission," Mol. Neurobiol 26 (2002) 2251 - 268. |
| Immunogen | GABAA Receptor a6 polyclonal antibody was raised against a peptide representing a sequence that is specific for the a6 subunit of the receptor. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| GABAA Receptor antibody recognizes 57k band in Western blots of rat brain. Peptide antigen has no homology with any other GABAA-R subunit. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB. | |
| Species Reactivity | Human,Rat |
| Concentration | 1mg/ml |
| Purification | Serum |
| Gene Symbol | Gabra6 |
|---|---|
| Alternative Names | Gabra-6 |
| Molecular Weight(MW) | 57 |
Application
GABA A Receptor alpha 6 Antibody| Application Notes | GABAA Receptor antibody recognizes 57k band in Western blots of rat brain. Peptide antigen has no homology with any other GABAA-R subunit. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB. |
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| Form | Liquid |
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| Storage Instructions | GABA A Receptor alpha 6 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
