Application
Western blot of rat cortex showing specific immunolabeling of the ~45k GABAA γ 2 protein phosphorylated at Ser327 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).| Product Name | GABAA Receptor (phospho Ser327) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2 |
| Product description | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gamma;s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. It has recently been suggested that PKCΕ regulates the sensitivity of GABAA alpha1beta2gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2 (Qi et al., 2007)1) Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser327 of the GABAA receptor, y2 subunit. |
| Modification | p-Ser327 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Species Reactivity | BovineChickenDogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Gabrg2 |
|---|---|
| Alternative Names | Gamma-aminobutyric acid receptor subunit gamma-2 GABA(A) receptor subunit gamma-2 |
| Molecular Weight(MW) | 45 |
| Tissue Specificity | Specific for ~45k GABAA receptor γ2 subunit phosphorylated at Ser327. Immunolabeling of the GABAA band is completely blocked by λ-phosphatase treatment. |
Application
Western blot of rat cortex showing specific immunolabeling of the ~45k GABAA γ 2 protein phosphorylated at Ser327 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | GABAA Receptor (Ser327) antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
