Application
Western blot of rat brain lysates from wild type (Control) and alpha3-knockout (alpha3-K/O) animals showing specific immunolabeling of the ~51k alpha3-subunit of the GABAA receptor. The labeling was absent from a lysate prepared from alpha3-knockout animals.| Product Name | GABAA Receptor Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gabra-3, |
| Product description | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).1) McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor α1-subtype. Nature Neurosci 3:587-592. |
| Immunogen | Fusion protein from N-terminal region of the a3-subunit of rat GABAA receptor. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500~1:1000 IHC:1:50~1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Gabra3 |
|---|---|
| Alternative Names | Gabra-3 |
| Molecular Weight(MW) | 51 |
Application
Western blot of rat brain lysates from wild type (Control) and alpha3-knockout (alpha3-K/O) animals showing specific immunolabeling of the ~51k alpha3-subunit of the GABAA receptor. The labeling was absent from a lysate prepared from alpha3-knockout animals.| Application Notes | WB:1:500~1:1000 IHC:1:50~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GABAA Receptor antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
