Application
Western blot of rat cerebellar (Cb) and hippocampal (Hipp) lysates showing specific immunolabeling of the ~55k beta1-subunit of the GABAA receptor.| Product Name | GABAA Receptor Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GARB1, Gabrb-1, |
| Product description | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).1) Brandon NJ, Jovanovic JN, Colledge M, Kittler JT, Brandon JM, Scott JD, Moss SJ (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor β-subunits. Mol Cell Neurosci 22:87-97. |
| Immunogen | Fusion protein from the cytoplasmic loop of the b1-subunit of rat GABAA receptor. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate, Xenopus, and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
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| Species Reactivity | BovineDogHumanMouseRatXenopusZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Gabrb1 |
|---|---|
| Alternative Names | GARB1 Gabrb-1 |
| Molecular Weight(MW) | 55 |
Application
Western blot of rat cerebellar (Cb) and hippocampal (Hipp) lysates showing specific immunolabeling of the ~55k beta1-subunit of the GABAA receptor.| Application Notes | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate, Xenopus, and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
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| Form | Liquid |
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| Storage Instructions | GABAA Receptor antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
