Application
Western blot of rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibo| Product Name | GABAB Receptor (phospho Ser783) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gpr51, Gpr51, G-protein coupled receptor 51, GABA-B receptor 2 |
| Product description | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).1) Kuramoto N, Wilkins ME, Fairfax BP, Revilla-Sanchez R, Terunuma M, Tamaki K, Iemata M, Warren N, Couve A, Calver A, Horvath Z, Freeman K, Carling D, Huang L, Gonzales C, Cooper E, Smart TG, Pangalos MN, Moss SJ (2007) Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron 53:233-247. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser783 of GABAB R2. |
| Modification | p-Ser783 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, non-human primate, chicken, bovine and Xenopus tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|
| Species Reactivity | BovineChickenHumanMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Gabbr2 |
|---|---|
| Alternative Names | Gpr51 Gpr51 G-protein coupled receptor 51 GABA-B receptor 2 |
| Molecular Weight(MW) | 102 |
Application
Western blot of rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibo| Application Notes | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, non-human primate, chicken, bovine and Xenopus tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GABAB Receptor antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
